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| Villarreal,LYB; Brandão,PEB; Chacón,JLV; Doretto Junior,L; Ito,N; Gama,NS; Ishizuka,MM; Luchese,A; Buchala,F; Astolfi-Ferreira,CS; Ferreira,AJP. |
| Avian Infectious Laryngotracheitis, caused by Infectious Laryngotracheitis Virus (ILTV), has been reported for decades in Brazilian laying and broiler flocks. More recently, outbreaks have occurred in São Paulo State. This study reports the application of PCR and DNA sequencing targeted to the p32 gene of ILTV using laying chicken samples from Bastos, São Paulo, Brazil. Three out of four field samples were positive by PCR. DNA sequencing of two samples evidenced homology of the amplified fragments with the p32 gene of ILTV. The results definitely confirmed the presence of ILTV in the birds during the outbreak. Further studies are needed to establish the sources of infection and to determine whether the detected virus was originated from vaccine or field... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Avian infectious laryngotracheitis; Brazil; P32; PCR; Sequencing. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2004000400011 |
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| Torre,D De la; Nuñez,LF; Parra,SHS; Astolfi-Ferreira,CS; Ferreira,AJP. |
| ABSTRACT This study determined the presence of Tremovirus A as the possible agent related to Avian Encephalomyelitis in broiler chicks from the states of São Paulo (SP) Paraná (PR), Goiás (GO), Santa Catarina (SC) and Rio Grande do Sul (RS), between the years 2006 and 2015. Samples of the nervous, digestive, respiratory, immune, and renal systems, plus muscular organs from broiler chicks with neurological problems such as ataxia and muscle tremors, and four (4) commercial vaccines as positive control, were tested by reverse-transcriptase (RT-PCR) amplification and DNA sequencing. A highly conserved region (P1) of the viral genome, was used to amplify a segment which encodes a structural protein VP4. Out of 112 samples, 46 were positive (42%) for Tremovirus... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chicken; Tremovirus A; Avian Encephalomyelitis; Detection; Molecular characterization. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000300527 |
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| Villarreal,LYB; Assayag,MS; Brandão,PE; Chacón,JLV; Bunger,AND; Astolfi-Ferreira,CS; Gomes,CR; Jones,RC; Ferreira,AJP. |
| This article reports a survey on turkey astrovirus (TAstV) and turkey coronavirus (TCoV) infections with RT-PCR in 17 turkey flocks affected by acute enteritis and two apparently normal turkey flocks located in the Southeastern region of Brazil by PCR (TAstV and TCoV). Seven out of the 17 affected flocks were positive for TAstV and 14 for TCoV, with seven co-infections. In one of the two apparently normal flocks, a TAstV-TCoV co-infection was found. Although a definitive association of these agents and the signs can not be made, the implications of these findings are discussed. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Astrovirus; Coronavirus; Turkey; Enteritis; Diagnosis. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2006000200010 |
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| Torre,D De la; Nuñez,LFN; Puga,B; Parra,SHS; Astolfi-Ferreira,CS; Ferreira,AJP. |
| ABSTRACT Enteric diseases affect poultry and cause important economic losses in many countries worldwide. Avian parvovirus has been linked to enteric conditions, such as malabsorption and runting-stunting syndrome (RSS), characterized by diarrhoea, and reduced weight gain and growth retardation. In 2013 and 2016, 79 samples were collected from different organs of chickens in Ecuador that exhibited signs of diarrhea and stunting syndrome, and analysed for the presence of chicken parvovirus (ChPV). The detection method of ChPV applied was Polymerase Chain Reaction (PCR), using primers designed from the conserved region of the viral genome that encodes the non-structural protein NS1. Out of the 79 samples, 50.6% (40/79) were positive for ChPV, and their... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chicken parvovirus; Enteric diseases; Molecular diagnostic; PCR. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000400643 |
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